Application
ICC/IF analysis of methanol-fixed HeLa cells using c-Myc (phospho Thr358) antibody (red) and DAPI (blue).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr358) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HT29 cells untreated (lane 1) or treated with UV (lane 2) using Myc (Phospho-Thr358) Antibody| Product Name | c-Myc (phospho Thr358) antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to Myc (Phospho Thr358)Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 358 (R-R-T(p)-H-N) derived from Human Myc. |
| Modification | p-Thr358 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MRTL bHLHe39 c-Myc MYC avian myelocytomatosis viral oncogene homolog myc-related translation/localization regulatory factor class E basic helix-loop-helix protein 39 transcription factor p64 proto-oncogene c-Myc myc proto-oncogene protein v-myc |
|---|---|
| Target | Myc (Phospho Thr358) |
| Molecular Weight(MW) | 60 (note) |
Application
ICC/IF analysis of methanol-fixed HeLa cells using c-Myc (phospho Thr358) antibody (red) and DAPI (blue).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr358) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29 cells untreated (lane 1) or treated with UV (lane 2) using Myc (Phospho-Thr358) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
