Application
Western blot analysis of extracts from Hela cells using Myc (Phospho-Thr58) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr58) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | c-Myc (phospho Thr58) antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to Myc (Phospho Thr58)Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from Human Myc. |
| Modification | p-Thr58 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MRTL bHLHe39 c-Myc MYC avian myelocytomatosis viral oncogene homolog myc-related translation/localization regulatory factor class E basic helix-loop-helix protein 39 transcription factor p64 proto-oncogene c-Myc myc proto-oncogene protein v-myc a |
|---|---|
| Target | Myc (Phospho Thr58) |
| Molecular Weight(MW) | 60 (note) |
Application
Western blot analysis of extracts from Hela cells using Myc (Phospho-Thr58) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr58) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
