Application
c-Myc antibody [N1], N-term detects c-Myc protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Myc protein stained by c-Myc antibody [N1], N-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | c-Myc antibody , N-term |
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| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to c-Myc (v-myc myelocytomatosis viral oncogene homolog (avian))The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human c-Myc. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cat, Pig, Rabbit, Sheep, Rhesus Monkey, ChimpanzeeMouse (100%), Rat (100%), Dog (100%), Cat (100%), Pig (100%), Rabbit (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | v-myc myelocytomatosis viral oncogene homolog (avian) MYC MRTL bHLHe39 cmyc |
|---|---|
| Target | c-Myc |
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Nucleus |
Application
c-Myc antibody [N1], N-term detects c-Myc protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Myc protein stained by c-Myc antibody [N1], N-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | NT2D1 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
