Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CNOT3 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using CNOT3 antibody at 1:100 dilution.| Product Name | CNOT3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CNOT3 (CCR4-NOT transcription complex, subunit 3)The CCR4-NOT complex functions as general transcription regulation complex. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CNOT3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, RabbitMouse (98%), Rat (98%), Xenopus laevis (92%), Rabbit (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0691 antibody LENG2 antibody NOT3 antibody NOT3H antibody CNOT3 antibody leukocyte receptor cluster member 2 antibody CCR4-NOT transcription complex subunit 3 antibody CCR4-associated factor 3 antibody "NOT3 (negative regulator of transcription |
|---|---|
| Molecular Weight(MW) | 82 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CNOT3 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using CNOT3 antibody at 1:100 dilution.| Positive Control | NT2D1 , IMR32 , Jurkat , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
