Application
Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 ug/ml.| Product Name | CNOT4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CNOT4CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCR4NOT transcription complex subunit4 CCR4-NOT transcription complex subunit 4 NOT4 NOT4H CNOT4 CCR4NOT transcription complex subunit 4 |
|---|---|
| Target | CNOT4 |
| Tissue Specificity | This antibody is specific for CNOT4 |
Application
Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85379-PEP - CNOT4 Peptide Lysates: Cat. No. GTX27910 - A549 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
