Application
Immunofluorescence staining of methanol-fixed Hela cells using cofilin (Phospho-Ser3) Antibody .Application
Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).Application
Western blot analysis of extracts from COLO205 cells using cofilin (Phospho-Ser3) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Phospho-Ser3) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | cofilin (Phospho Ser3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to cofilin (Phospho Ser3)Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. |
| Immunogen | Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin. |
| Modification | p-Ser3 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CFL CFL1 cofilin non-muscle isoform 18 kDa phosphoprotein p18 cofilin-1 cofilin 1 (non-muscle) |
|---|---|
| Target | cofilin (Phospho Ser3) |
| Molecular Weight(MW) | 19 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using cofilin (Phospho-Ser3) Antibody .
Application
Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Application
Western blot analysis of extracts from COLO205 cells using cofilin (Phospho-Ser3) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Phospho-Ser3) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
