Application
IHC-P image using - Detection of Cofilin by IHC-P in human breast carcinoma tissue.Application
Western blot analysis of extracts from COLO205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody ( Lane 3 and 4)| Product Name | Cofilin (phospho Ser3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cofilin ( phospho S3 )Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their ‘pointed’ (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility. |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-S^P-G-V). |
| Modification | p-Ser3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| IHC-P: Use at a dilution of 1/50-1/100. WB: Use at a dilution of 1/1,000. Detects a band of approximately 20 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 601442 CFL Cofilin P23528 CFL1 1072 Cofilin (phospho S3) Cofilin (phospho Ser3) Cofilin (pS3) Cofilin (pSer3) Cofilin phospho S3 Cofilin phospho Ser3 Phospho Cofilin (S3) Phospho Cofilin (Ser3) Phospho Cofilin (pS3) Phospho Cofilin (pSer3) |
|---|---|
| Target | Cofilin |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
IHC-P image using - Detection of Cofilin by IHC-P in human breast carcinoma tissue.
Application
Western blot analysis of extracts from COLO205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody ( Lane 3 and 4)| Positive Control | MDCK cells treated with staurosporine; Jurkat cells treated with hydrogen peroxide |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/50-1/100. WB: Use at a dilution of 1/1,000. Detects a band of approximately 20 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.3, 50% Glycerol and 150mM NaCl containing 0.02% sodium azide |
