Application
Sample (30 ug of whole cell lysate) A: H1299 B: Molt-4 12% SDS PAGE diluted at 1:1000Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Product Name | Cofilin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cofilin 2 (muscle) (cofilin 2 (muscle))Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cofilin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineZebrafish (89%), Xenopus laevis (87%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NEM7 antibody CFL2 antibody "cofilin muscle isoform antibody" cofilin-2 antibody cofilin 2 (muscle) antibody |
|---|---|
| Molecular Weight(MW) | 19 kDa (note) (3) |
| Cellular Localization | Nucleus matrix , Cytoplasm , cytoskeleton |
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Molt-4 12% SDS PAGE diluted at 1:1000
Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
