Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 5% SDS-PAGE Collagen III antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III antibody at 1:200 dilution.Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 5% SDS-PAGE Collagen III antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. Mouse tissue extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. A. 50 µg rat liver lysate/extract 5% SDS-PAGE COL3A1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | COL3A1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COL3A1 (collagen, type III, alpha 1)This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human COL3A1. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Chicken, Rhesus Monkey, BovinePig (94%), Chicken (83%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EDS4A antibody FLJ34534 antibody COL3A1 antibody alpha1 (III) collagen antibody Ehlers-Danlos syndrome type IV autosomal dominant antibody collagen fetal antibody collagen alpha-1(III) chain antibody collagen type III alpha 1 antibody |
|---|---|
| Molecular Weight(MW) | 139 kDa (note) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 5% SDS-PAGE Collagen III antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III antibody at 1:200 dilution.
Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 5% SDS-PAGE Collagen III antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. Mouse tissue extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. A. 50 µg rat liver lysate/extract 5% SDS-PAGE COL3A1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro2A , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , rat liver , Mouse ESC , PC-12 , Rat2 |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
