Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody diluted at 1:500.Application
COL4A1 antibody detects COL4A1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. COL4A1 antibody diluted at 1:500.| Product Name | COL4A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COL4A1 (collagen, type IV, alpha 1)This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus of human COL4A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (88%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | POREN1 collagen type IV alpha 1 ICH HANAC arresten |
|---|---|
| Target | COL4A1 |
| Molecular Weight(MW) | 161 kDa (note) (2) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix , basement membrane |
Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody diluted at 1:500.
Application
COL4A1 antibody detects COL4A1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. COL4A1 antibody diluted at 1:500.| Positive Control | SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
