Application
Collagen II antibody detects Collagen II protein by western blot analysis. Human plasma and HepG2 conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody diluted at 1:500.| Product Name | Collagen II antibody , N-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Collagen II (collagen, type II, alpha 1)This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Collagen II. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Rabbit, Chicken, BovineMouse (92%), Rat (96%), Xenopus laevis (88%), Dog (100%), Rabbit (100%), Chicken (95%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANFH antibody AOM antibody COL11A3 antibody MGC131516 antibody SEDC antibody COL2A1 antibody cartilage collagen antibody collagen alpha-1(II) chain antibody chondrocalcin antibody "collagen II alpha-1 polypeptide antibody" alpha-1 type II collag |
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| Molecular Weight(MW) | 142 kDa (note) (2) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Collagen II antibody detects Collagen II protein by western blot analysis. Human plasma and HepG2 conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody diluted at 1:500.| Positive Control | HepG2 sup. |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
