Application
Western blot analysis of extracts from HT29 and HepG2 cells using Connexin 43(Ab-367) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HT29 and HepG2 cells using Connexin 43 Antibody .| Product Name | Connexin 43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Connexin 43One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. |
| Immunogen | Peptide sequence around aa. 365~369 (R-A-S-S-R) derived from Human Connexin 43. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CX43 DFNB38 GJAL HSS ODDD GJA1 connexin 43 gap junction alpha-1 protein gap junction 43 kDa heart protein connexin-43 gap junction protein alpha 1 43kDa |
|---|---|
| Target | Connexin 43 |
| Molecular Weight(MW) | 43 (note) |
Application
Western blot analysis of extracts from HT29 and HepG2 cells using Connexin 43(Ab-367) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29 and HepG2 cells using Connexin 43 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
