Application
ICC/IF analysis of IEC-6 cells using Connexin 43 antibody at a dilution of 1:200 (red) and Hoechst 33342 (blue).Application
WB analysis of rat heart membranes using Connexin-43 antibody at a dilution of 1:400 in the (1) absence or (2) presence of blocking peptide.Application
WB analysis of mouse brain membranes using Connexin-43 antibody at a dilution of 1:400 in the (1) absence or (2) presence of blocking peptide.Application
IHC-Fr analysis of rat cerebellum using Connexin 43 antibody at a dilution of 1:300 (red) and DAPI (blue). Mol: molecular layer. G: granule layer.| Product Name | Connexin 43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Connexin 43This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| ICC/IF:1:200~1:500 IHC-F:1:50~1:100 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ODDD HLHS1 AVSD3 GJA1 DFNB38 CX43 HSS GJAL gap junction protein alpha 1 43kDa |
|---|---|
| Target | Connexin 43 |
Application
ICC/IF analysis of IEC-6 cells using Connexin 43 antibody at a dilution of 1:200 (red) and Hoechst 33342 (blue).
Application
WB analysis of rat heart membranes using Connexin-43 antibody at a dilution of 1:400 in the (1) absence or (2) presence of blocking peptide.
Application
WB analysis of mouse brain membranes using Connexin-43 antibody at a dilution of 1:400 in the (1) absence or (2) presence of blocking peptide.
Application
IHC-Fr analysis of rat cerebellum using Connexin 43 antibody at a dilution of 1:300 (red) and DAPI (blue). Mol: molecular layer. G: granule layer.| Application Notes | ICC/IF:1:200~1:500 IHC-F:1:50~1:100 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. |
| Storage Buffer | PBS, pH 7.4, 1% BSA, 0.05% NaN3. |
