Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using COPB antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using COPB antibody at 1:200 dilution.| Product Name | COPB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COPB (coatomer protein complex, subunit beta 1)This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COPB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, BovineMouse (99%), Rat (99%), Zebrafish (97%), Xenopus laevis (96%), Pig (100%), Chicken (98%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | COPB antibody DKFZp761K102 antibody FLJ10341 antibody FLJ46444 antibody FLJ57957 antibody COPB1 antibody beta-coat protein antibody coatomer subunit beta antibody beta coat protein antibody beta-cop antibody "coatomer protein complex subunit bet |
|---|---|
| Molecular Weight(MW) | 107 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasmic vesicle , COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , Golgi apparatus membrane , Cytoplasmic vesicle , COPI-coa |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using COPB antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using COPB antibody at 1:200 dilution.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
