Application
Sample(30 ug whole cell lysate) A:H1299 B:Hep G2 7.5% SDS PAGE diluted at 1:500Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COPD antibody diluted at 1:2000.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPD antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.| Product Name | COPD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COPD (archain 1)This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COPD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (98%), Rat (98%), Xenopus laevis (83%), Chicken (92%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COPD antibody ARCN1 antibody "coatomer protein complex subunit delta antibody" coatomer protein delta-COP antibody delta-COP antibody archain vesicle transport protein 1 antibody coatomer subunit delta antibody coatomer delta subunit antibody delt |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasmic vesicle , COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , Golgi apparatus membrane , Cytoplasmic vesicle , COPI-coa |
Application
Sample(30 ug whole cell lysate) A:H1299 B:Hep G2 7.5% SDS PAGE diluted at 1:500
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COPD antibody diluted at 1:2000.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPD antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
