Application
Western blot using Cortactin [pY421] Polyclonal Antibody, Rabbit| Product Name | Cortactin (phospho Tyr421) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cortactin (phospho Tyr421)Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks. |
| Immunogen | Synthetic peptide (Mouse) derived from the region of mouse cortactin that contains tyrosine 421. |
| Modification | p-Tyr421 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 83 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Ems1 1110020L01Rik Cortactin 13043 Cttn Cortactin (phospho Y421) Cortactin (phospho Tyr421) Cortactin (pY421) Cortactin (pTyr421) Cortactin phospho Y421 Cortactin phospho Tyr421 Phospho Cortactin (Y421) Phospho Cortactin (Tyr421) Phospho Corta |
|---|---|
| Target | Cortactin (phospho Tyr421) |
| Cellular Localization | Cytoplasmic |
Application
Western blot using Cortactin [pY421] Polyclonal Antibody, Rabbit| Positive Control | 5HD47, a C3H 10T1/2 cell line overexpressing Src and transfected with mouse Cortactin |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 83 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4 |
