| Product Name | Cortactin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CortactinCortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. |
| Immunogen | Synthetic peptide corresponding to the N-terminal region of human cortactin (amino acids 18-35) conjugated to KLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/500, this concentration is determined using the mouse fibroblast NIH3T3 cell line. WB: Use at a dilution of 1/1000, this is determined using a rat brain cytosol extract, a whole extract of chicken embryo fibroblasts, or a whole cell extract of the human epidermoid carcinoma A431 cell line. The antibody recognizes human, rat, mouse, and chicken cortactin, (65-75 kDa, 2-3 bands). Staining of cortactin is specifically inhibited with cortactin immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | 164765 CTTN EMS1 Q14247 2017 Cortactin |
|---|---|
| Target | Cortactin |
| Cellular Localization | Cytoplasmic |
| Positive Control | Human epidermoid carcinoma, A431 cell line for Western blotting, the mousefibroblasts NIH3T3 cell line for immunofluorescence |
|---|---|
| Application Notes | IF: Use at a dilution of 1/500, this concentration is determined using the mouse fibroblast NIH3T3 cell line. WB: Use at a dilution of 1/1000, this is determined using a rat brain cytosol extract, a whole extract of chicken embryo fibroblasts, or a whole cell extract of the human epidermoid carcinoma A431 cell line. The antibody recognizes human, rat, mouse, and chicken cortactin, (65-75 kDa, 2-3 bands). Staining of cortactin is specifically inhibited with cortactin immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage, please keep at -20°C . Avoid repeared freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM sodium azide.Constituents: 0.01M PBS, pH 7.4 |
