Application
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4 antibody at 1:100 dilution.Application
Sample(30 µg of whole cell lysate) A:A431 B:MOLT4 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg of COX4 antibody . Western blot analysis was performed using COX4 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | COX4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX4 (cytochrome c oxidase subunit IV isoform 1)Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, Chimpanzee, BovineRhesus Monkey (92%), Chimpanzee (100%), Bovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COX4 antibody, COX4-1 antibody, COXIV antibody, FLJ23483 antibody, MGC72016 antibody, COX4I1 antibody, "cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody", COX IV-1 antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) (2) |
| Cellular Localization | Mitochondrion inner membrane |
Application
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4 antibody at 1:100 dilution.
Application
Sample(30 µg of whole cell lysate) A:A431 B:MOLT4 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg of COX4 antibody . Western blot analysis was performed using COX4 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
