Application
Immunofluorescence analysis of methanol-fixed A431, using COX5A antibody at 1:200 dilution.Application
COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. COX5A antibody [N1C3] diluted at 1:500.Application
COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. COX5A antibody [N1C3] diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000Application
Sample(30 ug whole cell lysate) A:HeLa S3 B:Hep G2 15% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using COX5A antibody at 1:500 dilution.| Product Name | COX5A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX5A (cytochrome c oxidase subunit Va)Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Rhesus Monkey, Chimpanzee, BovineRat (93%), Xenopus laevis (86%), Dog (94%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | COX antibody, COX-VA antibody, VA antibody, COX5A antibody, "cytochrome c oxidase subunit 5A, mitochondrial antibody", mitochondrial cytochrome c oxidase subunit Va antibody, cytochrome c oxidase polypeptide Va antibody, "cytochrome c oxidase polypeptide, |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane |
Application
Immunofluorescence analysis of methanol-fixed A431, using COX5A antibody at 1:200 dilution.
Application
COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. COX5A antibody [N1C3] diluted at 1:500.
Application
COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. COX5A antibody [N1C3] diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000
Application
Sample(30 ug whole cell lysate) A:HeLa S3 B:Hep G2 15% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using COX5A antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
