Application
Sample(30 μg of whole cell lysate) A:Hep G2 15% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using COX5B antibody at 1:100 dilution.| Product Name | COX5B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX5B (cytochrome c oxidase subunit Vb)Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COX5B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, BovineMouse (85%), Rat (82%), Dog (83%), Pig (83%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COXVB antibody, COX5B antibody, "cytochrome c oxidase subunit 5B, mitochondrial antibody", "cytochrome c oxidase polypeptide VB, mitochondrial antibody", cytochrome c oxidase subunit Vb antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa (note) |
Application
Sample(30 μg of whole cell lysate) A:Hep G2 15% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using COX5B antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
