Application
COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: COX6A2 protein stained by COX6A2 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample(30 µg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. COX6A2 antibody [N3C3] diluted at 1:500.| Product Name | COX6A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX6A2 (cytochrome c oxidase subunit VIa polypeptide 2)Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX6A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COX6AH antibody, COXVIAH antibody, COX6A2 antibody, cytochrome c oxidase polypeptide VIa-heart antibody, "cytochrome c oxidase subunit 6A2, mitochondrial antibody", cytochrome c oxidase subunit VIA-muscle antibody, COX VIa-M antibody, cytochrome c oxidase |
|---|---|
| Molecular Weight(MW) | 11 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane |
Application
COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: COX6A2 protein stained by COX6A2 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample(30 µg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. COX6A2 antibody [N3C3] diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
