Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CPT1B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | CPT1B antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CPT1B (carnitine palmitoyltransferase 1B (muscle))The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and one of the transcripts is bicistronic. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CPT1B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, Rhesus Monkey, BovineRat (88%), Pig (95%), Sheep (95%), Rhesus Monkey (98%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CPT1-M antibody CPT1M antibody CPTI antibody CPTI-M antibody FLJ55729 antibody FLJ58750 antibody KIAA1670 antibody M-CPT1 antibody MCCPT1 antibody MCPT1 antibody CPT1B antibody carnitine palmitoyltransferase I-like protein antibody "carnitine O |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) |
| Cellular Localization | Mitochondrion outer membrane |
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CPT1B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
