Application
Sample(30 ug whole cell lysate) A:H1299 B:HeLa S3 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Creatine kinase 1B antibody at 1:300 dilution.| Product Name | Creatine kinase 1B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Creatine kinase 1B (creatine kinase, mitochondrial 1B)Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Creatine kinase 1B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rat, Xenopus laevis, Rabbit, BovineRat (98%), Xenopus laevis (88%), Rabbit (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CKMT antibody CKMT1 antibody CKMT1A antibody UMTCK antibody CKMT1B antibody "creatine kinase U-type mitochondrial antibody" "creatine kinase mitochondrial 1 (ubiquitous) antibody" ubiquitous mitochondrial creatine kinase antibody U-MtCK antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side |
Application
Sample(30 ug whole cell lysate) A:H1299 B:HeLa S3 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Creatine kinase 1B antibody at 1:300 dilution.| Positive Control | 293T , H1299 , HeLaS3 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
