Application
Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract. Detection occurs using a 1:1,000 dilution of antibody followed by a 1:5,000 dilution of HRP Goat anti Rabbit IgG with visualization via ECL| Product Name | CREB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREBcyclic AMP responsive element binding protein (CREB) is a ubiquitous transcription factor regulated by changes in cyclic AMP (cAMP) levels. CREB binds as a homodimer to CRE sites (cAMP response elements) and belongs to the basic leucine zipper family of DNA binding proteins. Increases in cAMP levels result in the specific phosphorylation of CREB by protein kinase A. Phosphorylated CREB interacts with the transcriptional co-activator CBP (CREB binding protein or p265). It has recently been demonstrated that CREB phosphorylation correlates with light regulated circadian rhythms. |
| Immunogen | CREB-1 (p43) peptide corresponding to a region near the N-terminus of the human protein, conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAGSAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CREB1 |
|---|---|
| Target | CREB |
| Tissue Specificity | A partial cross-reactivity is observed against CREM-1 protein. |
Application
Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract. Detection occurs using a 1:1,000 dilution of antibody followed by a 1:5,000 dilution of HRP Goat anti Rabbit IgG with visualization via ECL| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
