Application
Western blot analysis of extracts from 293, HT29 and Hela cells using CREB(Ab-133) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-133) Antibody (left) or the same antibody preincubated with blocking peptide(right).Application
Western blot analysis of extracts from 293, HT29 and Hela cells using CREB Antibody .| Product Name | CREB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREBThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. |
| Immunogen | Peptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CREB MGC9284 CREB1 cyclic AMP-responsive element-binding protein 1 active transcription factor CREB transactivator protein CREB-1 cAMP-response element-binding protein-1 cAMP-responsive element-binding protein 1 cAMP responsive element binding pr |
|---|---|
| Target | CREB |
| Molecular Weight(MW) | 43 (note) |
Application
Western blot analysis of extracts from 293, HT29 and Hela cells using CREB(Ab-133) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-133) Antibody (left) or the same antibody preincubated with blocking peptide(right).
Application
Western blot analysis of extracts from 293, HT29 and Hela cells using CREB Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
