Application
CREB antibody detects CREB protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CREB antibody dilution: 1:500.Application
CREB antibody detects CREB protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. CREB antibody dilution: 1:500.Application
CREB antibody detects CREB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREB protein stained by CREB antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Application
CREB antibody detects CREB protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CREB antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CREB antibody detects CREB protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: CREB protein stained by CREB antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
CREB antibody detects CREB protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CREB antibody diluted at 1:500.Application
CREB antibody detects CREB protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. CREB antibody diluted at 1:500.Application
CREB antibody detects CREB protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CREB antibody dilution: 1:500.| Product Name | CREB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREB (cAMP responsive element binding protein 1)This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CREB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineXenopus laevis (94%), Pig (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CREB1 cAMP responsive element binding protein 1 |
|---|---|
| Target | CREB |
| Molecular Weight(MW) | 35 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
CREB antibody detects CREB protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CREB antibody dilution: 1:500.
Application
CREB antibody detects CREB protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. CREB antibody dilution: 1:500.
Application
CREB antibody detects CREB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREB protein stained by CREB antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application
CREB antibody detects CREB protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CREB antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CREB antibody detects CREB protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: CREB protein stained by CREB antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
CREB antibody detects CREB protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CREB antibody diluted at 1:500.
Application
CREB antibody detects CREB protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. CREB antibody diluted at 1:500.
Application
CREB antibody detects CREB protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CREB antibody dilution: 1:500.| Positive Control | Jurkat , Jurkat nuclear , *human CREB1-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
