Application
Western blot analysis of extracts from Hela and HT29 cells using CREB(Ab-129) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela and HT29 cells using CREB Antibody .| Product Name | CREB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREBCREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories . |
| Immunogen | Peptide sequence around aa.127~131 (I-L-S-R-R) derived from Human CREB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CREB MGC9284 CREB1 cyclic AMP-responsive element-binding protein 1 active transcription factor CREB transactivator protein CREB-1 cAMP-response element-binding protein-1 cAMP-responsive element-binding protein 1 cAMP responsive element binding pr |
|---|---|
| Target | CREB |
| Molecular Weight(MW) | 43 (note) |
Application
Western blot analysis of extracts from Hela and HT29 cells using CREB(Ab-129) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela and HT29 cells using CREB Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
