Application
Immunofluorescence staining of methanol-fixed Hela cells using Rel (Phospho-Ser503) Antibody| Product Name | c-Rel (phospho Ser503) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Rel (Phospho Ser503)The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 (PubMed 18037997)). |
| Immunogen | Peptide sequence around phosphorylation site of serine 503 (T-S-S(p)-D-S) derived from Human Rel. |
| Modification | p-Ser503 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| Suggested starting dilutions are as follows. ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C-Rel REL proto-oncogene c-Rel v-rel avian reticuloendotheliosis viral oncogene homolog oncogene REL avian reticuloendotheliosis C-Rel proto-oncogene protein v-rel reticuloendotheliosis viral oncogene homolog (avian) |
|---|---|
| Target | Rel (Phospho Ser503) |
| Molecular Weight(MW) | 78 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Rel (Phospho-Ser503) Antibody| Application Notes | Suggested starting dilutions are as follows. ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
