Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CRM1 antibody at 1:250 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CRM1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CRM1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CRM1 (exportin 1 (CRM1 homolog, yeast))The protein encoded by this gene mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CRM1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (99%), Rat (99%), Xenopus laevis (96%), Chicken (99%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRM1 antibody, DKFZp686B1823 antibody, emb antibody, XPO1 antibody, exportin-1 (required for chromosome region maintenance) antibody, "CRM1, yeast, homolog antibody", "exportin 1 (CRM1, yeast, homolog) antibody", exportin-1 antibody, chromosome region main |
|---|---|
| Molecular Weight(MW) | 123 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , nucleoplasm , Cajal body , nucleolus |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CRM1 antibody at 1:250 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CRM1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
