Application
Left panel: human CTR1 staining of breast cancer tissue. Right: human CTR1-antigen competition in breast cancer tissue.Application
Immunohistochemistry: SLC31A1/CTR1 Antibody [ - The photo on the left is antibody staining of hCTR1 overexpressing cell line. The photo on the right is antibody plus peptide competition staining.Application
Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody [ - CTR1 antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red)| Product Name | CTR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CTR1 (Copper Transporter Receptor 1)The trace metal copper (Cu) plays a crucial role in mammalian cells as a cofactor for many enzymes. Cu-related genetic diseases, such as Menkes disease and Wilson disease, arise from a lack of Cu homeostasis in mammalian cells. CTR1 is a high-affinity copper-uptake protein. The C-terminal domain is similar to the Raf family of protein kinases, but itis first two-thirds encodes a novel protein domain. CTR1 provides the primary avenue for copper uptake in mammalian cells, thereby, affecting Cu homeostasis and embryonic development. |
| Immunogen | A synthetic peptide derived from a C-terminal sequence of the human CTR1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-P |
| This antibody may be used for immunohistochemistry (formalin-fixed paraffin-embedded tissue). This antibody has not proven useful for Western Blot. No other applications have been determined.Suggested working dilutions: *Western Blot (-)Immunohistochemistry - 1:250Immunofluorescence - ND*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SLC31A1 hCTR1 O15431 COPT1 1317 603085 MGC75487 CTR1 |
|---|---|
| Target | CTR1 |
| Tissue Specificity | This antibody is specific for human CTR1 |
Application
Left panel: human CTR1 staining of breast cancer tissue. Right: human CTR1-antigen competition in breast cancer tissue.
Application
Immunohistochemistry: SLC31A1/CTR1 Antibody [ - The photo on the left is antibody staining of hCTR1 overexpressing cell line. The photo on the right is antibody plus peptide competition staining.
Application
Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody [ - CTR1 antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red)| Positive Control | Human breast cancer and kidney tissue |
|---|---|
| Application Notes | This antibody may be used for immunohistochemistry (formalin-fixed paraffin-embedded tissue). This antibody has not proven useful for Western Blot. No other applications have been determined.Suggested working dilutions: *Western Blot (-)Immunohistochemistry - 1:250Immunofluorescence - ND*The investigator should determine the optimal working dilution for a specific application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Antisera containing 0.1% sodium azide |
