Application
Immunofluorescence of CTRP2 in Jurkat cells with CTRP2 antibody at 20 μg/mL.Application
Western blot of recombinant CTRP2: (A) 5 ng, (B) 25 ng, and (C) 50 ng with CTRP2 at 1 ug/ml.Application
Immunocytochemistry of CTRP2 in Jurkat cells with CTRP2 antibody at 10 ug/ml.| Product Name | CTRP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CTRP2Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are are expressed in a wide variety of tissues (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). Of the CTRPs, CTRP2 is most similar structurally and functionally to adiponectin. Recombinant CTRP2 rapidly activated AMPK and MAPK in cultured C2C12 cells, leading to increased glycogen accumulation and fatty acid oxidation (3). |
| Immunogen | Recombinant mouse CTRP2 (Genbank accession No. AAH30324). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTRP2 |
|---|---|
| Target | CTRP2 |
| Tissue Specificity | This antibody is specific for CTRP2 |
Application
Immunofluorescence of CTRP2 in Jurkat cells with CTRP2 antibody at 20 μg/mL.
Application
Western blot of recombinant CTRP2: (A) 5 ng, (B) 25 ng, and (C) 50 ng with CTRP2 at 1 ug/ml.
Application
Immunocytochemistry of CTRP2 in Jurkat cells with CTRP2 antibody at 10 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85071-PEP - CTRP2 Peptide Lysates: Cat. No. GTX27899 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
