Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CUGBP2 antibody at 1:500 dilution.Application
CUGBP2 antibody detects CUGBP2 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUGBP2 protein stained by CUGBP2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG 7.5% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000Application
CUGBP2 antibody detects CUGBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CUGBP2 antibody diluted at 1:500.| Product Name | CUGBP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CUGBP2 (CUGBP, Elav-like family member 2)Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CUGBP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, ChickenRat (89%), Zebrafish (85%), Xenopus laevis (91%), Chicken (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRUNOL3 antibody, CUGBP2 antibody, ETR-3 antibody, ETR3 antibody, NAPOR antibody, CELF2 antibody, CUGBP Elav-like family member 2 antibody, ELAV-type RNA-binding protein 3 antibody, RNA-binding protein BRUNOL-3 antibody, hNAPOR antibody, "CUG triplet repea |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (32) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CUGBP2 antibody at 1:500 dilution.
Application
CUGBP2 antibody detects CUGBP2 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CUGBP2 protein stained by CUGBP2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG 7.5% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
CUGBP2 antibody detects CUGBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CUGBP2 antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
