Application
Western Blot analysis of CXCL1 expression in transfected 293T cell line by CXCL1 polyclonal antibody. A: Non-transfected lysate. B: CXCL1 transfected lysate. 15% SDS PAGE diluted at 1:500| Product Name | CXCL1 / GRO alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GRO alpha (chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha))Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CXC chemokines are further subdivided into ELR and non-ELR types based on the presence or absence of a glu-leu-arg sequence adjacent and N terminal to the CXC motif. ELR types are chemotactic for neutrophils, while non-ELR types are chemotactic for lymphocytes.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRO alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FSP antibody GRO1 antibody GROa antibody MGSA antibody MGSA-a antibody NAP-3 antibody SCYB1 antibody CXCL1 antibody GRO1 oncogene (melanoma growth-stimulating activity) antibody melanoma growth stimulatory activity alpha antibody "GRO1 oncogene ( |
|---|---|
| Target | GRO alpha |
| Molecular Weight(MW) | 11 kDa (note) |
| Cellular Localization | Secreted |
Application
Western Blot analysis of CXCL1 expression in transfected 293T cell line by CXCL1 polyclonal antibody. A: Non-transfected lysate. B: CXCL1 transfected lysate. 15% SDS PAGE diluted at 1:500| Positive Control | GRO alpha tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
