Application
Western blot analysis of CXCR4 in HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/mL.Application
Flow cytometry analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.Application
Immunofluorescence of CXCR4 in HeLa cells with CXCR4 antibody at 20 ug/mL.Application
Immunocytochemistry of CXCR4 in HeLa cells with CXCR4 antibody at 2 ug/mL.| Product Name | CXCR4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CXCR4This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Immunogen | CXCR4 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human CXCR4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIPWB |
| WB: 1 - 2 ug/ml. IP: 10 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml. FACS: 0.1 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD184 FB22 LCR1 WHIM D2S201E NPYRL chemokine (C-X-C motif) receptor 4 LESTR LAP3 NPYR HSY3RR HM89 NPY3R NPYY3R |
|---|---|
| Target | CXCR4 |
| Tissue Specificity | CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members. |
Application
Western blot analysis of CXCR4 in HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/mL.
Application
Flow cytometry analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.
Application
Immunofluorescence of CXCR4 in HeLa cells with CXCR4 antibody at 20 ug/mL.
Application
Immunocytochemistry of CXCR4 in HeLa cells with CXCR4 antibody at 2 ug/mL.| Application Notes | WB: 1 - 2 ug/ml. IP: 10 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml. FACS: 0.1 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
