Application
CXCR4 antibody detects CXCR4 protein by western blot analysis. Non-transfected (-) and -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody at a dilution of 1:1000.| Product Name | CXCR4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CXCR4 (chemokine (C-X-C motif) receptor 4)This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | WHIM NPY3R NPYR NPYY3R HM89 HSY3RR FB22 LAP3 NPYRL D2S201E CD184 chemokine (C-X-C motif) receptor 4 LESTR LCR1 A |
|---|---|
| Target | CXCR4 |
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Cell membrane |
Application
CXCR4 antibody detects CXCR4 protein by western blot analysis. Non-transfected (-) and -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody at a dilution of 1:1000.| Positive Control | human CXCR4 -transfected 293T (unboiled) |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
