Application
Western blot analysis of Cyclin B1 (pS126) expression in HT29 nocodazole-treated (A); Jurkat (B); HeLa (C); Raw264.7 (D) whole cell lysates.Application
Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Cyclin B1 (phospho Ser126) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin B1 (phospho Ser126)The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary. |
| Modification | p-Ser126 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCNB CCNB1 cyclin B1 |
|---|---|
| Target | Cyclin B1 |
| Tissue Specificity | Recognizes endogenous levels of Cyclin B1 (pS126) protein. |
Application
Western blot analysis of Cyclin B1 (pS126) expression in HT29 nocodazole-treated (A); Jurkat (B); HeLa (C); Raw264.7 (D) whole cell lysates.
Application
Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
