Application
Western blot analysis of extracts from HepG2 and HT29 cells using Cyclin B1 (Ab-147) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1 Antibody .| Product Name | Cyclin B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin B1The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| Immunogen | Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCNB CCNB1 G2/mitotic-specific cyclin-B1 G2/mitotic-specific cyclin B1 cyclin B1 |
|---|---|
| Target | Cyclin B1 |
| Molecular Weight(MW) | 60 (note) |
Application
Western blot analysis of extracts from HepG2 and HT29 cells using Cyclin B1 (Ab-147) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
