Application
Application| Product Name | Cyclin B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cyclin B1During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9). |
| Immunogen | Synthetic peptide: TLPQLNSALVQDLAKAVAKV, corresponding to C terminal amino acids 414-433 of Human Cyclin B1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| For IHC: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | CCNB1 Cyclin B1 891 123836 P14635 CCNB |
|---|---|
| Target | Cyclin B1 |
| Tissue Specificity | Cyclin B1 |
Application
Application| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | For IHC: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin |
