Application
Breast carcinoma stained withApplication
Human Breast Carcinoma stained with anti-Cyclin B1 antibody| Product Name | Cyclin B1 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cyclin B1 predilutedIn mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. |
| Immunogen | Synthetic peptide (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| For IHC-P: Ready-to-use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCNB1 Cyclin B1 891 123836 P14635 CCNB |
|---|---|
| Target | Cyclin B1 |
| Cellular Localization | Cytoplasmic |
Application
Breast carcinoma stained with
Application
Human Breast Carcinoma stained with anti-Cyclin B1 antibody| Positive Control | Jurkat cells and breast carcinoma |
|---|---|
| Application Notes | For IHC-P: Ready-to-use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | Contains sodium azide |
