Application
Western blot analysis of extract of various cells, using CCND2 antibody.Application
Immunofluorescence analysis of U2OS cell using CCND2 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded mouse brain using CCND2 antibody at dilution of 1:100 (40x lens).Application
Immunohistochemistry of paraffin-embedded human stomach using CCND2 antibody at dilution of 1:100 (40x lens).| Product Name | Cyclin D2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin D2The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014] |
| Immunogen | Recombinant protein of human CCND2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cyclin D2 CCND2 MPPH3 KIAK0002 |
|---|---|
| Target | Cyclin D2 |
| Molecular Weight(MW) | 33 kDa (note) |
Application
Western blot analysis of extract of various cells, using CCND2 antibody.
Application
Immunofluorescence analysis of U2OS cell using CCND2 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded mouse brain using CCND2 antibody at dilution of 1:100 (40x lens).
Application
Immunohistochemistry of paraffin-embedded human stomach using CCND2 antibody at dilution of 1:100 (40x lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
