Application
Western blot analysis of extracts from Mouse heart tissue using Cyclin D3 (Phospho-Thr283) Antibody. The lane on the left is treated with the antigen-specific peptide.| Product Name | Cyclin D3 (phospho Thr283) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin D3 (phospho Thr283)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 283 (T-S-T(p)-P-T) derived from Human Cyclin D3. |
| Modification | p-Thr283 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | cyclin D3 CCND3 |
|---|---|
| Target | Cyclin D3 (phospho Thr283) |
| Tissue Specificity | The antibody detects endogenous level of Cyclin D3 only when phosphorylated at Threonine 283. |
Application
Western blot analysis of extracts from Mouse heart tissue using Cyclin D3 (Phospho-Thr283) Antibody. The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
