Application
Human Breast Carcinoma stained with anti-Cyclin E antibodyApplication
staining Cyclin E in breast carcinoma by Immunohistochemistry (FFPE-sections).| Product Name | Cyclin E antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cyclin E predilutedCyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in Cyclin E protein have been implicated as indicators of worse prognosis in various cancers. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Ready to use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mMcitrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCNE1 CCNE P24864 898 123837 Cyclin E |
|---|---|
| Target | Cyclin E |
| Cellular Localization | Nuclear |
Application
Human Breast Carcinoma stained with anti-Cyclin E antibody
Application
staining Cyclin E in breast carcinoma by Immunohistochemistry (FFPE-sections).| Positive Control | SKBR3 cells, MAD109 cells, breast carcinoma and placenta |
|---|---|
| Application Notes | IHC-P: Ready to use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mMcitrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
