Application
Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1 (phospho-Thr395) Antibody .| Product Name | Cyclin E1 (phospho Thr395) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin E1 (Phospho Thr395)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1 |
| Modification | p-Thr395 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCNE CCNE1 cyclin Es G1/S-specific cyclin-E1 cyclin Et cyclin E1 |
|---|---|
| Target | Cyclin E1 (Phospho Thr395) |
| Molecular Weight(MW) | 48 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1 (phospho-Thr395) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
