Application
Western blot using GeneTex's affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).| Product Name | Cyclin E2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin E2Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions.Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition. Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCNE2 |
|---|---|
| Target | Cyclin E2 |
Application
Western blot using GeneTex's affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
