Application
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:500.Application
Immunofluorescence analysis of methanol-fixed A431, using PPIF antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPIF antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:5000.| Product Name | Cyclophilin F antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclophilin F (peptidylprolyl isomerase F)The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclophilin F. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus Monkey, BovineXenopus laevis (82%), Chicken (95%), Rhesus Monkey (99%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYP3 antibody Cyp-D antibody FLJ90798 antibody MGC117207 antibody PPIF antibody rotamase F antibody "peptidyl-prolyl cis-trans isomerase mitochondrial antibody" cyclophilin 3 antibody "peptidyl-prolyl cis-trans isomerase F mitochondrial antibody" |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:500.
Application
Immunofluorescence analysis of methanol-fixed A431, using PPIF antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPIF antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000
Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with PPIF antibody [N1C3] diluted at 1:5000.| Positive Control | 293T , A431 , mouse heart , rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
