Application
WB analysis of extracts from various cell lines using CYP11A1 antibody.Application
ICC/IF analysis of MCF7 cells using CYP11A1 antibody (green) and DAPI (blue).| Product Name | CYP11A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CYP11A1This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human CYP11A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYPXIA1 CYP11A P450SCC cytochrome P450 family 11 subfamily A polypeptide 1 |
|---|---|
| Target | CYP11A1 |
| Molecular Weight(MW) | 60 kDa (note) |
Application
WB analysis of extracts from various cell lines using CYP11A1 antibody.
Application
ICC/IF analysis of MCF7 cells using CYP11A1 antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
