Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:10000Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.| Product Name | CYP17A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP17A1 (cytochrome P450, family 17, subfamily A, polypeptide 1)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP17A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (91%), Chimpanzee (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CPT7 antibody CYP17 antibody P450C17 antibody S17AH antibody CYP17A1 antibody cytochrome P450c17 antibody cytochrome P450 17A1 antibody cytochrome P450-C17 antibody "cytochrome P450 subfamily XVII (steroid 17-alpha-hydroxylase) adrenal hyperplasi |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) |
| Cellular Localization | Membrane , Membrane; Single-pass membrane protein , Membrane , Membrane |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:10000
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.
Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.| Positive Control | NIH-3T3 , C2C12 , MCF-7 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
