Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using CYP26A1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP26A1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYP26A1 antibody at 1:500 dilution.| Product Name | CYP26A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP26A1 (cytochrome P450, family 26, subfamily A, polypeptide 1)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP26A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, Rhesus Monkey, BovineRat (92%), Pig (97%), Chicken (83%), Rhesus Monkey (100%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CP26 antibody CYP26 antibody P450RAI antibody P450RAI1 antibody CYP26A1 antibody cytochrome P450 26A1 antibody cytochrome P450RAI antibody "P450 retinoic acid-inactivating 1 antibody" retinoic acid 4-hydroxylase antibody "cytochrome P450 subfam |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane , Microsome membrane |
Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using CYP26A1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP26A1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYP26A1 antibody at 1:500 dilution.| Positive Control | 293T , mouse liver , mouse kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
