Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE CYP27A1 antibody diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded hela xenograft, using CYP27A1 antibody antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse Liver 7.5% SDS PAGE diluted at 1:1000| Product Name | CYP27A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP27A1 (cytochrome P450, family 27, subfamily A, polypeptide 1)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP27A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Rabbit, Rhesus MonkeyPig (80%), Rabbit (82%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CP27 antibody CTX antibody CYP27 antibody CYP27A1 antibody "sterol 26-hydroxylase mitochondrial antibody" "5-beta-cholestane-3-alpha 7-alpha 12-alpha-triol 26-hydroxylase antibody" sterol 27-hydroxylase antibody vitamin D(3) 25-hydroxylase antibo |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (2) |
| Cellular Localization | Mitochondrion membrane |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE CYP27A1 antibody diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded hela xenograft, using CYP27A1 antibody antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse Liver 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , H1299 , HeLa , HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
