Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CYP3A43 antibody at 1:250 dilution.Application
CYP3A43 antibody detects CYP3A43 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP3A43 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CYP3A43 antibody detects CYP3A43 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP3A43 protein stained by CYP3A43 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | CYP3A43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP3A43 (cytochrome P450, family 3, subfamily A, polypeptide 43)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, it is unknown whether the enzyme is also involved in xenobiotic metabolism. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP3A43. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC119315 antibody MGC119316 antibody CYP3A43 antibody "cytochrome P450 subfamily IIIA polypeptide 43 antibody" cytochrome P450 3A43 antibody cytochrome P450 polypeptide 43 antibody "cytochrome P450 family 3 subfamily A polypeptide 43 antibody" |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane , Microsome membrane |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CYP3A43 antibody at 1:250 dilution.
Application
CYP3A43 antibody detects CYP3A43 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP3A43 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CYP3A43 antibody detects CYP3A43 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP3A43 protein stained by CYP3A43 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
